Structure of maltoporin from Salmonella typhimurium ligated with a nitrophenyl-maltotrioside.

MALTOPORIN WAS CRYSTALLIZED BY HANGING-DROP METHOD. DROP: 5-8 MG/ML PROTEIN, 0.3% C8E4, 0.8% C6DAO, 1 MM CACL2, 1MM MGCL2, 14-18% PEG 1500, 0.02% NAN3 RESERVOIR: 28-32% PEG 1500, vapor diffusion - hanging drop

ALL SIDE CHAIN ATOMS WITHOUT DENSITY WERE ASSIGNED ZERO OCCUPANCY.

B-VALUES OF WILSON PLOT WAS CALCULATED WITH THE CCP4 PROGRAM WILSON.

THROUGHOUT EXCEPT FOR VERY LAST ROUND (REFINEMENT AGAINST ALL REFLECTIONS)

MEMBRANE PROTEIN, SUGAR TRANSPORT, SPECIFIC PORIN, BETA BARREL