Crystal structure of a HD-GYP domain (a cyclic-di-GMP phosphodiesterase) containing a tri-nuclear metal centre

Oxford Protein Production Facility (OPPF)

c-di-GMP; Cyclic diguanosine monophosphate

Crystal structure of an HD-GYP domain cyclic-di-GMP phosphodiesterase reveals an enzyme with a novel trinuclear catalytic iron centre.

0.9 M succinate pH 7, 2% PEG 2000, 0.1 M MES buffer pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 277K

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT

asymmetric unit contains one dimer, which is the biological assembly

Structural Genomics, Oxford Protein Production Facility, OPPF, phosphohydrolase, HYDROLASE