Crystal structure of SeMet SznF from Streptomyces achromogenes var. streptozoticus NRRL 2697

An N-nitrosating metalloenzyme constructs the pharmacophore of streptozotocin.

Streptomyces achromogenes subsp. streptozoticus

0.1 M Tris, pH 8.5, 0.1 M magnesium chloride, 15-30% w/v PEG4000

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

native SznF with on-path intermediate bound in cupin domain

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. Restrained refinement against unmerged SAD data directly, using 10 TLS refinement cycles with 3 TLS groups for molecules A & D and 4 groups for molecules B & C (isotropic temperature factors) followed by 10 cycles of maximum likelihood restrained refinement using 0.04 weighting term and jelly-body refinement with sigma 0.025, plus automatically generated local NCS restraints.

non-heme iron-dependent, cupin domain, helix bundle, monooxygenase, N-nitrosation, metal-bound, anaerobic, SeMet, selenemethionine, OXIDOREDUCTASE