Crystal structure of SznF from Streptomyces achromogenes var. streptozoticus NRRL 2697 with a bound N(delta)-hydroxy-N(omega)-methyl-L-arginine intermediate

An N-nitrosating metalloenzyme constructs the pharmacophore of streptozotocin.

Streptomyces achromogenes subsp. streptozoticus

0.1 M Tris, pH 8.5, 0.1 M magnesium chloride, 14-20% w/v PEG4000

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. 10 cycles of TLS refinement using isotropic temperature factors (11 groups for each molecule of a.u.) followed by 10 cycles of maximum likelihood restrained refinement with 0.07 weighting term, 0.035 jelly-body refinement and automatically generated local NCS restraints.

non-heme iron-dependent, cupin domain, helix bundle, monooxygenase, N-nitrosation, L-arginine derivative, metal-bound, ligand-bound, anaerobic, OXIDOREDUCTASE