Crystal Structure of the G359F (small subunit) Point Mutant of Carbamoyl Phosphate Synthetase

Carbamoyl-phosphate synthetase. Creation of an escape route for ammonia

LEU H 216 HAS WRONG CHIRALITY AT ATOM CA

carbamoyl phosphate synthetase large chain

carbamoyl-phosphate synthetase small chain

Carbamoyl-phosphate synthetase ammonia chain

Carbamoyl-phosphate synthetase glutamine chain

PEG-8000, manganese chloride, potassium chloride, ADP, ornithine, tetraethylammonium chloride, HEPPS, pH 7.4, batch, temperature 277K

SEQUENCE
THE CLONE USED VARIED FROM THE SWISS-PROT
ENTRY AT RESIDUE 46 OF CHAINS ACEG (SWS P00968)
AND RESIDUE 183 OF CHAINS BDFH (SWS P00907).

Biological assembly is an (ab)4 hetero-tetramer: Comprised of chains A,B,C,D,E,F,G,H