DNA Polymerase Lambda, TTP:At Mg2+ Product State Ternary Complex, 960 min

Watching right and wrong nucleotide insertion captures hidden polymerase fidelity checkpoints.

DNA (5'-D(*CP*GP*GP*CP*AP*GP*TP*AP*CP*TP*G)-3')

Pol Lambda,DNA polymerase beta-2,Pol beta2,DNA polymerase kappa

80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG

National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)

Time-Lapse Crystallography, DNA Polymerase Lambda, Double Strand Break Repair, DNA Synthesis Fidelity, REPLICATION