Influenza B polymerase bound to four heptad repeats of serine 5 phosphorylated Pol II CTD

Structural basis of an essential interaction between influenza polymerase and Pol II CTD.

N-terminal His-tag C-terminal linker and TEV site

N-terminal linker C-terminal linker and TEV site
RNA-directed RNA polymerase catalytic subunit

RNA (5'-R(*UP*AP*UP*AP*CP*CP*UP*CP*UP*GP*CP*UP*UP*C)-3')

RNA (5'-R(P*AP*GP*UP*AP*GP*UP*AP*AP*CP*AP*AP*GP*AP*G)-3')

Four heptad repeats of serine 5 phosphorylated Pol II CTD

FluB polymerase in a buffer containing 500 mM NaCl, 50 mM Hepes pH 7.5, 5% glycerol and 2mM Tris(2-carboxyethyl)phosphine (TCEP) was mixed with 5' and 3' ends of vRNA promoter and CTD peptide. Reservoir solution contained 0.1 M bicine pH 9.0, 10% MPD.

Polymerase acidic protein, RNA-directed RNA polymerase catalytic subunit, Polymerase basic protein 2, DNA-directed RNA polymerase subunit/RNA Complex
influenza B virus RNA-dependent RNA polymerase, vRNA promoter, Pol II serine 5 phosphorylated CTD peptide, viral protein