4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID

N-acetyl-beta-D-glucosamine; 2-acetamido-2-deoxy-beta-D-glucose; 2-acetamido-2-deoxy-D-glucose; 2-acetamido-2-deoxy-glucose; N-ACETYL-D-GLUCOSAMINE

Structure basis for AA98 inhibition on the activation of endothelial cells mediated by CD146.

CD146, Cell surface glycoprotein P1H12,Melanoma cell adhesion molecule,Melanoma-associated antigen A32,Melanoma-associated antigen MUC18,S-endo 1 endothelial-associated antigen

0.1M Hepes, pH 7.5, 8~15% w/v Polyethylene glycol 2000 MME

National Natural Science Foundation of China (NSFC)

AA98 Fab is purified to homogeneity and then pooled with purified CD146 D4-D5 protein. Elution profiles of AA98 Fab, CD146 D4-D5 and complex of both are compared. Same result could also be found in the final crystal structure.

AA98 Fab heavy chain, AA98 Fab light chain, Cell surface glycoprotein MUC18