Structure of Dimethylformamidase, tetramer

A 2-Tyr-1-carboxylate Mononuclear Iron Center Forms the Active Site of a Paracoccus Dimethylformamidase.

Comparison of CryoEM and X-ray structures of dimethylformamidase

CTF correction was done with Relion, during refinement and reconstruction

A total of 25 frames were saved from the 60 second exposure, resulting in ~1.08 electron/frame

Two rounds of 2D classification was performed prior to angular assignment and reconstruction

Glow discharge was carried out in Quorum Glocube instrument at 25mA for 90 seconds.

Sample at higher salt exists mostly as tetramer

Blot force was 0 and blotting was done for 3.5 seconds

Department of Biotechnology (DBT, India)

Science and Engineering Research Board (SERB)

At low concentration, the molecules can disassemble to form dimer containing 2x alpha+beta

N,N-dimethylformamidase large subunit (E.C.3.5.1.56), N,N-dimethylformamidase small subunit (E.C.3.5.1.56)
ab polypeptide, mononuclear iron, amidohydrolase, tetramer, HYDROLASE