Ruminococcus bromii Amy12-D392A with maltoheptaose

Structure and substrate recognition by the Ruminococcus bromii amylosome pullulanases.

8.75 - 8.9 mg/ml of protein and 10 mM maltoheptaose via hanging drop against a well solution containing 16% PEG 3350, 4% glycerol, 0.3 ammonium acetate, and 0.1 M Bis-Tris pH 6.5.

United States - Israel Binational Science Foundation (BSF)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED

GH13, pullulanase, CBM48, complex, HYDROLASE