APE1 phosphorothioate substrate complex with abasic ribonucleotide DNA

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

[(2~{R},3~{S},4~{S})-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-sulfanyl-phosphinic acid

Altered APE1 activity on abasic ribonucleotides is mediated by changes in the nucleoside sugar pucker.

DNA-(apurinic or apyrimidinic site) lyase

DNA (5'-D(*GP*GP*AP*TP*CP*CP*GP*TP*CP*GP*AP*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3')

DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*C)-R(P*(YA4))-D(P*CP*GP*AP*CP*GP*GP*AP*TP*CP*C)-3')

APEX1, APEX nuclease,APEN,Apurinic-apyrimidinic endonuclease 1, APE-1, REF-1, Redox factor-1

50 mM tri-Sodium Citrate (pH 4), 100 mM NaCl, 10% Peg 6,000

National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS)

DNA Repair, Abasic Ribonucleotide, AP-Endonuclease, LYASE, LYASE-DNA complex