X-ray crystal structure of AmpC WT beta-lactamase in complex with covalently bound imipenem

(5R)-5-[(1S,2R)-1-formyl-2-hydroxypropyl]-3-[(2-{[(E)-iminomethyl]amino}ethyl)sulfanyl]-4,5-dihydro-1H-pyrrole-2-carboxylic acid
IMIPENEM, open form; N-FORMIMIDOYL-THIENAMYCINE, open form

Structural Basis for Imipenem Inhibition of Class C beta-lactamases

1.7 M potassium phosphate; crystal was then soaked in saturated imipenem for 40 minutes, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 296K

AmpC WT beta-lactamase covalently bound to moxalactam

TEM-1 beta-lactamase covalently bound to imipenem

Residues 284-290 of chain A were not seen in the electron density.

beta-lactamase, carbapenem, imipenem, HYDROLASE