Structure of a photobleached state of IrisFP under high intensity laser-light

Structural evidence for a two-regime photobleaching mechanism in a reversibly switchable fluorescent protein.

Green to red photoconvertible GPF-like protein EosFP

2.1M ammonium sulfate, 0.1M bicine, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystal structure of IrisFP in its green state (chromophore in cis conformation)

Crystal structure of IrisFP in its dark state (chromophore in trans conformation)

Crystal structure of IrisFP in its red state(chromophore in cis conformation)

Crystal structure of native IrisFP (low X-ray dose, planar chromophore)

Crystal structure of IrisFP in its blinked state (distorted chromophore)

PDB ENTRY 2VVH - IRISFP FLUORESCENT PROTEIN IN ITS GREEN FORM, CIS CONFORMATION

Fluorescent protein, Photobleaching, Beta-Barrel, Decarboxylation