Zoogloea ramigera biosynthetic thiolase Q183Y/Y218E mutant

Engineering potassium activation into biosynthetic thiolase.

Acetoacetyl-CoA thiolase,Beta-ketothiolase

0.44 M (NH4)2SO4, 1.76 M Li2SO4, 0.1 M sodium citrate pH 5.5, 1 mM DTT, 1 mM EDTA, 1 mM NaN3

acetyl-CoA C-acetyltransferase, acetoacetyl-CoA thiolase, type II thiolase, biosynthetic thiolase, potassium activation, TRANSFERASE

(chain A and ((resid 3 and (name N or name CA or name C or name O or name CB )) or resid 4 through 165 or (resid 166 through 167 and (name N or name CA or name C or name O or name CB )) or resid 168 or (resid 169 through 171 and (name N or name CA or name C or name O or name CB )) or resid 172 through 174 or (resid 175 through 177 and (name N or name CA or name C or name O or name CB )) or resid 178 through 184 or (resid 185 and (name N or name CA or name C or name O or name CB or name CG or name CD or name CE )) or resid 186 or (resid 187 and (name N or name CA or name C or name O or name CB or name CG )) or resid 188 through 195 or (resid 196 and (name N or name CA or name C or name O or name CB )) or resid 197 through 204 or (resid 205 and (name N or name CA or name C or name O or name CB )) or resid 206 or resid 210 through 212 or (resid 213 and (name N or name CA or name C or name O or name CB )) or resid 214 through 217 or (resid 218 and (name N or name CA or name C or name O or name CB or name CG )) or resid 219 through 220 or (resid 221 and (name N or name CA or name C or name O or name CB )) or (resid 222 through 229 and (name N or name CA or name C or name O or name CB )) or (resid 230 and (name N or name CA or name C or name O or name CB or name CG )) or resid 231 through 240 or (resid 241 and (name N or name CA or name C or name O or name CB )) or resid 242 through 305 or (resid 306 and (name N or name CA or name C or name O or name CB )) or resid 307 through 327 or (resid 328 and (name N or name CA or name C or name O or name CB )) or resid 329 or (resid 330 and (name N or name CA or name C or name O or name CB )) or resid 331 through 391 or resid 723))

(chain B and ((resid 3 and (name N or name CA or name C or name O or name CB )) or resid 4 through 39 or (resid 40 and (name N or name CA or name C or name O or name CB or name CG )) or resid 41 through 137 or (resid 138 and (name N or name CA or name C or name O or name CB or name CG )) or resid 139 through 165 or (resid 166 through 167 and (name N or name CA or name C or name O or name CB )) or resid 168 or (resid 169 through 171 and (name N or name CA or name C or name O or name CB )) or resid 172 through 174 or (resid 175 through 177 and (name N or name CA or name C or name O or name CB )) or resid 178 through 184 or (resid 185 and (name N or name CA or name C or name O or name CB or name CG or name CD or name CE )) or resid 186 through 195 or (resid 196 and (name N or name CA or name C or name O or name CB )) or resid 197 through 210 or (resid 211 and (name N or name CA or name C or name O or name CB or name CG1)) or resid 212 or (resid 213 and (name N or name CA or name C or name O or name CB )) or resid 214 through 217 or (resid 218 and (name N or name CA or name C or name O or name CB or name CG )) or resid 219 through 220 or (resid 221 and (name N or name CA or name C or name O or name CB )) or (resid 222 through 229 and (name N or name CA or name C or name O or name CB )) or (resid 230 and (name N or name CA or name C or name O or name CB or name CG )) or resid 231 or (resid 232 and (name N or name CA or name C or name O or name CB )) or resid 233 through 235 or (resid 236 through 238 and (name N or name CA or name C or name O or name CB )) or resid 239 through 240 or (resid 241 and (name N or name CA or name C or name O or name CB )) or resid 242 through 297 or (resid 298 and (name N or name CA or name C or name O or name CB or name CG or name CD )) or resid 299 through 305 or (resid 306 and (name N or name CA or name C or name O or name CB )) or resid 307 through 327 or (resid 328 and (name N or name CA or name C or name O or name CB )) or resid 329 or (resid 330 and (name N or name CA or name C or name O or name CB )) or resid 331 through 391 or resid 724))

(chain C and ((resid 3 and (name N or name CA or name C or name O or name CB )) or resid 4 through 39 or (resid 40 and (name N or name CA or name C or name O or name CB or name CG )) or resid 41 through 137 or (resid 138 and (name N or name CA or name C or name O or name CB or name CG )) or resid 139 through 165 or (resid 166 through 167 and (name N or name CA or name C or name O or name CB )) or resid 168 or (resid 169 through 171 and (name N or name CA or name C or name O or name CB )) or resid 172 through 174 or (resid 175 through 177 and (name N or name CA or name C or name O or name CB )) or resid 178 through 186 or (resid 187 and (name N or name CA or name C or name O or name CB or name CG )) or resid 188 through 195 or (resid 196 and (name N or name CA or name C or name O or name CB )) or resid 197 through 204 or (resid 205 and (name N or name CA or name C or name O or name CB )) or resid 206 or resid 210 through 212 or (resid 213 and (name N or name CA or name C or name O or name CB )) or resid 214 through 220 or (resid 221 and (name N or name CA or name C or name O or name CB )) or (resid 222 through 229 and (name N or name CA or name C or name O or name CB )) or resid 230 through 231 or (resid 232 and (name N or name CA or name C or name O or name CB )) or resid 233 through 235 or (resid 236 through 238 and (name N or name CA or name C or name O or name CB )) or resid 239 through 240 or (resid 241 and (name N or name CA or name C or name O or name CB )) or resid 242 through 297 or (resid 298 and (name N or name CA or name C or name O or name CB or name CG or name CD )) or resid 299 through 305 or (resid 306 and (name N or name CA or name C or name O or name CB )) or resid 307 through 327 or (resid 328 and (name N or name CA or name C or name O or name CB )) or resid 329 or (resid 330 and (name N or name CA or name C or name O or name CB )) or resid 331 through 391 or resid 501))

(chain D and (resid 3 through 39 or (resid 40 and (name N or name CA or name C or name O or name CB or name CG )) or resid 41 through 137 or (resid 138 and (name N or name CA or name C or name O or name CB or name CG )) or resid 139 through 184 or (resid 185 and (name N or name CA or name C or name O or name CB or name CG or name CD or name CE )) or resid 186 or (resid 187 and (name N or name CA or name C or name O or name CB or name CG )) or resid 188 through 204 or (resid 205 and (name N or name CA or name C or name O or name CB )) or resid 206 or resid 210 or (resid 211 and (name N or name CA or name C or name O or name CB or name CG1)) or resid 212 through 217 or (resid 218 and (name N or name CA or name C or name O or name CB or name CG )) or resid 219 through 229 or (resid 230 and (name N or name CA or name C or name O or name CB or name CG )) or resid 231 through 297 or (resid 298 and (name N or name CA or name C or name O or name CB or name CG or name CD )) or resid 299 through 391 or resid 501))