Cryo-EM structure of RecBCD+DNA complex revealing activated nuclease domain

PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

Mechanism for nuclease regulation in RecBCD.

Ternary complex of RecBCD with forked DNA substrate and ADPNP

Glow discharge was used to thin the carbon film, but allowed to dissipate prior to use. Detergent or amphipols treatment was used to render carbon hydrophilic. See article for details.

Used at the end to apply local CTF corrections for individual particles

Residues 913-932 modelled as poly-Alanine in model to reflect uncertainty of the exact position of this loop due to weak density.
RecBCD enzyme subunit RecB,RecBCD enzyme subunit RecB,RecBCD enzyme subunit RecB

Exodeoxyribonuclease V 135 kDa polypeptide,Exodeoxyribonuclease V beta chain,Exonuclease V subunit RecB,ExoV subunit RecB,Helicase/nuclease RecBCD subunit RecB,Exodeoxyribonuclease V 135 kDa polypeptide,Exodeoxyribonuclease V beta chain,Exonuclease V subunit RecB,ExoV subunit RecB,Helicase/nuclease RecBCD subunit RecB,Exodeoxyribonuclease V 135 kDa polypeptide,Exodeoxyribonuclease V beta chain,Exonuclease V subunit RecB,ExoV subunit RecB,Helicase/nuclease RecBCD subunit RecB

Exodeoxyribonuclease V 125 kDa polypeptide,Exodeoxyribonuclease V gamma chain,Exonuclease V subunit RecC,ExoV subunit RecC

Exodeoxyribonuclease V 67 kDa polypeptide,Exodeoxyribonuclease V alpha chain,Exonuclease V subunit RecD,ExoV subunit RecD,Helicase/nuclease RecBCD subunit RecD

RecBCD enzyme subunit RecB, RecBCD enzyme subunit RecC, RecBCD enzyme subunit RecD/DNA Complex
Helicase, Nuclease, SH3, Homologous Recombination, hydrolase