Structure of an Rsp5xUbxSna3 complex: Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3

Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3.

CYTOSOLIC FRAGMENT (UNP residues 104-127)

0.1M Bis Tris Propane, 21% w/v PEG3350, 1% w/v polypropylene glycol, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K

E3 ubiquitin-protein ligase RSP5 (E.C.6.3.2.-), Protein SNA3, Ubiquitin

Ligase, E3, Rsp5, NEDD4, Ubiquitin, HECT, Sna3, thioester, maleimide, crosslink, Ligase-protein binding complex