Cryo-EM structure of PCV2 Replicase bound to ssDNA

Mechanism of DNA Interaction and Translocation by the Replicase of a Circular Rep-Encoding Single-Stranded DNA Virus.

Resolution is based on 3DFSC global value

20 mM HEPES, pH 8.2, 500 mM NaCl, 0.2 mM TCEP, 10 mM MgCl2

Sample was monodisperse according to size exclusion chromatography

4 uL samples were applied to the grids for 3 seconds and blotted using a blot force of 2, a blot time of 4 seconds, and a drain time of 0 seconds.

RepP, Replication-associated protein, PCV2 Replicase

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Rolling circle replication, CRESS-DNA virus, SF3 helicase, Porcine Circovirus, PCV2, REPLICATION, HYDROLASE-DNA complex