BG505 SOSIP.v5.2 N241/N289 in complex with the polyclonal Fab pAbC-2 from animal Rh.33311 (Wk26 time point)

N-acetyl-beta-D-glucosamine; 2-acetamido-2-deoxy-beta-D-glucose; 2-acetamido-2-deoxy-D-glucose; 2-acetamido-2-deoxy-glucose; N-ACETYL-D-GLUCOSAMINE

Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM.

28188 symmetry-expanded particles. See the Methods section in the manuscript for details.

Polyclonal complexes were generated by incubation of isolated polyclonal Fabs with recombinantly expressed BG505 SOSIP and subsequent SEC purification. Map and model were reconstructed from the immune complex dataset using the focused classification approach.

polyclonal Fab pAbC-2 from animal Rh.33311

Frames were aligned using MotionCorr and GCTF was applied for estimation of CTF parameters.

Polyclonal complexes were generated by incubation of isolated polyclonal Fabs with recombinantly expressed BG505 SOSIP and subsequent SEC purification.

Blotting time varied between 3 and 7 seconds.

HIV, vaccine design, BG505, VIRAL PROTEIN-IMMUNE SYSTEM complex, Polyclonal antibodies, EMPEM