A conserved two-step binding for the UAF1 regulator to the USP12 deubiquitinating enzyme.

Ubiquitin carboxyl-terminal hydrolase 12

Recombinant expression followed by chemical synthesis

Deubiquitinating enzyme 12,Ubiquitin thioesterase 12,Ubiquitin-hydrolyzing enzyme 1,Ubiquitin-specific-processing protease 12

USP1-associated factor 1,WD repeat endosomal protein,p80

3.2% PEG4000, 0.1mM MMT pH6.5, 0.1 mM TCEP.

Cryo- 30% Glycerol

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

WD repeat-containing protein 48, Unknown

DUB-Activator complex USP1 paralog, STRUCTURAL PROTEIN