Crystal structure of gamma-glutamyl hydrolase (C108A) from zebrafish

Structural insights into the hydrolysis and polymorphism of methotrexate polyglutamate by zebrafish gamma-glutamyl hydrolase

20%(w/v) PEG 8000, 0.2M magnesium chloride, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

The same protein complexed with MTX polyglutamate

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS