Human SUMO E1 complex with a SUMO1-AMP mimic

Active site remodelling accompanies thioester bond formation in the SUMO E1.

Ubiquitin-like 1-activating enzyme E1B, Anthracycline-associated resistance ARX

SUMO-1, Sentrin, Ubiquitin-like protein SMT3C, SMT3 homolog 3, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein UBL1, GAP-modifying protein 1, GMP1

0.1 MES, 24% PEG 3350, 1.3 M AMMONIUM ACETATE, 10 mM ATP, 10 mM MgCl2, 10 mM TCEP, 2.5% PEG 400, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K

cysteylglycylglycyl 5'-(sulfamoylaminodeoxy)adenosine

LIGAND CYS-GLY-GLY-AMSN, WHERE AMSN HAS ID JZU IN THIS FILE, WAS LINKED TO SUMO1 BY INTEIN-MEDIATED LIGATION TO MAKE CHAIN D.
CHAIN D IS COMPOSED AS UB/UBI-CYS-GLY-GLY-AMSN, ADENYLATE MIMIC.

E1, SUMO, UBIQUITIN, THIOESTER, ADENYLATION, INHIBITOR, ACYL-ADENYLATE INTERMEDIATE, Acetylation, Ligase, Nucleus, Phosphoprotein, Ubl conjugation pathway, ATP-binding, Nucleotide-binding, Polymorphism, Cytoplasm, Isopeptide bond, Membrane