Human transketolase in covalent complex with donor ketose D-xylulose-5-phosphate, crystal 2

Sub-angstrom-resolution crystallography reveals physical distortions that enhance reactivity of a covalent enzymatic intermediate.

Reservoir mixture of 13.5-15 % PEG 6000 (w/v), 4% PEG 400 (v/v), 2 % glycerol (v/v) , pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 279.0K

DX5 A1016 IS A HYDROLYZED D-XYULOSE-5-PHOSPHATE COVALENTLY LINKED TO TDP A1015

MOEWS & KRETSINGER, J.MOL.BIOL.91(1973)201-228

thiamin diphosphate, enzyme catalysis, pentose phosphate pathway, transferase