Directed Evolution of Transaminases By Ancestral Reconstruction. Using Old Proteins for New Chemistries

Reverse engineering: transaminase biocatalyst development using ancestral sequence reconstruction

150 plus 150 nL drops with protein at 10 mg/mL and reservoir conditions of 12% PEG 8000, 15% glycerol, 150 mM MgCl2, 100 mM Tris pH 7.1. Microseeds were used for nucleation of the crystals.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

phylogenetics, directed evolution, transaminase, protein engineering, TRANSFERASE