CRYSTAL STRUCTURE OF THYMIDINE KINASE FROM HERPES SIMPLEX VIRUS TYPE I COMPLEXED WITH 5-BROMOVINYLDEOXYURIDINE

Exploring the active site of herpes simplex virus type-1 thymidine kinase by X-ray crystallography of complexes with aciclovir and other ligands.

3'-Amino Thymidine Affinity Matrix for the Purification of Herpes Simplex Virus Thymidine Kinase

Crystal Structures of the Thymidine Kinase from Herpes Simplex Virus Type-1 in Complex with Deoxythymidine and Ganciclovir

The Complete DNA Sequence of the Long Unique Region in the Genome of Herpes Simplex Virus Type 1

Purification and Crystallization of Thymidine Kinase from Herpes Simplex Virus Type 1

Nucleotide Sequence of the Thymidine Kinase Gene of Herpes Simplex Virus Type 1

Herpes simplex virus (type 1 / strain 17)

HANGING-DROP: 8ML PROTEIN SOLUTION PLUS 4ML PRECIPITATING SOLUTION EQUILIBRATED AGAINST PRECIPITATING SOLUTION AT 25 C. PROTEIN SOLUTION: HSV-TK 1.0MG/ML; 40MM TRIS-CL PH 7.5; 3MM DTT; 0.2MM DT. PRECIPITATING SOLUTION: 30% SATURATED AMMONIUM SULPHATE; 200MM TRIS-CL PH 6.75; 3MM DTT; 0.2 MM DT. EXCHANGE OF DEOXYTHYMIDINE BOUND TO THE PROTEIN FOR 5-BROMOVINYLDEOXYURIDINE WAS MADE BY WASHING THE CRYSTALS FIVE TIMES IN A 3 ML SOLUTION (33% SATURATED AMMONIUM SULPHATE; 100MM TRIS-CL PH 6.75) CONTAINING 5-BROMOVINYLDEOXYURIDINE (BVDU)., vapor diffusion - hanging drop

NCS RESTRAINTS NOT USED IN FINAL CYCLES.

IN C-TERMINAL PORTIONS OF MODELLED SEGMENTS, PEPTIDE HAS BECOME SIGNIFICANTLY NON-PLANAR.

MIR + NCS IMPROVEMENT AND SOLVENT FLATTENING FOR ORIGINAL NATIVE, 1KIN

THYMIDINE KINASE, 5-BROMOVINYLDEOXYURIDINE

PHOSPHOTRANSFERASE, THYMIDINE KINASE, VIRIDAE, DS-DNA ENVELOPED VIRUSES, HERPESVIRIDAE, ALPHAHERPESVIRINAE, SUBSTRATE ANALOG, 5-BROMOVINYLDEOXYURIDINE