Toxoplasma gondii NTPDase1 C258S/C268S E493G crystallized with Mg and AMPNP

Adenosine 5-(alpha,beta-imido)diphosphate

The ATP/ADP substrate specificity switch between Toxoplasma gondii NTPDase1 and NTPDase3 is caused by an altered mode of binding of the substrate base.

NTPase-II, Nucleoside triphosphate hydrolase 2, Nucleoside-triphosphatase II

100 mM Tris (pH 8.8), 50 mM MgCl2, 25% (v/v) pentaerythritol propoxylate (17/8 PO/OH), 10 mM AMPNP, 10 mM magnesium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 292K

THE ELECTRON DENSITY INDICATES BINDING OF THE ADENINE BASE OF AMPNP IN A PUTATIVE TGNTPDASE3 ADP-PRODUCT BINDING MODE. HOWEVER, DUE TO THE WEAK DENSITY THE OCCUPANCY OF THE LIGAND WAS SET TO 0.

Nucleoside-triphosphatase 2 (E.C.3.6.1.15)