Poliovirus polymerase elongation complex (r4_form)

Structures of coxsackievirus, rhinovirus, and poliovirus polymerase elongation complexes solved by engineering RNA mediated crystal contacts.

RNA (5'-R(*AP*AP*GP*UP*CP*UP*CP*CP*AP*GP*GP*UP*CP*UP*CP*UP*CP*GP*GP*AP*AP*A)-3')

RNA (5'-R(P*UP*GP*UP*UP*CP*CP*GP*AP*GP*AP*GP*A)-3')

RNA (5'-R(*GP*GP*GP*AP*GP*AP*UP*GP*A)-3')

0.16 M MgCl2, 0.08 M Tris-HCl, 24% (w/v) PEG 4000, 20% (v/v) glycerol and directly frozen for data collection , VAPOR DIFFUSION, SITTING DROP, temperature 289K

polymerase, RNA-dependent RNA polymerase, protein-RNA complex, transferase-rna complex