S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate

Backbone structure of a small helical integral membrane protein: A unique structural characterization.

ligation independent cloning vector producing N-terminal His6 tag

2-acrylamido-2-methyl-1-propanesulfonic acid

1 mM [U-100% 15N] Rv1761c, 10 % D2O, 90 % H2O, 20 mM sodium acetate, 150 mM DPC, 5 % polyacrylamide gel, 90% H2O/10% D2O

1 mM [U-100% 15N] Rv1761c, 10 % D2O, 90 % H2O, 20 mM sodium acetate, 150 mM DPC, 4.4 % polyacrylamide gel, 1.1 % 2-acrylamido-2-methyl-1-propanesulfonic acid, 90% H2O/10% D2O

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax

Schwieters, Kuszewski, Tjandra and Clore

PROTEIN, integral membrane protein, MEMBRANE PROTEIN