Recognition dynamics up to microseconds revealed from RDC derived ubiquitin ensemble in solution

Recognition dynamics up to microseconds revealed from an RDC-derived ubiquitin ensemble in solution.

molecular dynamics simulated annealing ensemble refinement

0.9 mM [U-100% 13C; U-100% 15N] ubiquitin, 50 mM sodium phosphate, 90% H2O/10% D2O

0.6 mM [U-100% 13C; U-100% 15N] ubiquitin, 50 mM sodium phosphate, 90% H2O/10% D2O

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax

de Groot, van Aalten, Scheek, Amadei, Vriend and Berendsen

ubiquitin, RDC, residual dipolar coupling, Cytoplasm, Nucleus, Ubl conjugation, SIGNALING PROTEIN