Ternary complex of D256A mutant of DNA Polymerase Beta

2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE

Amino Acid Substitution in the Active Site of DNA Polymerase beta Explains the Energy Barrier of the Nucleotidyl Transfer Reaction.

DNA (5'-D(*CP*CP*GP*AP*CP*AP*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3')

DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*C)-3')

50 mM Imidazole, 350 mM Sodium Acetate, 18 % PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K

Nucleotidyl Transfer, dNTP, Non-hydrolyzable analog, Transferase-DNA complex