Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Lys27Met mutant of Charybdotoxin

NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
2'-MONOPHOSPHOADENOSINE 5'-DIPHOSPHORIBOSE

(1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate
1-Palmitoyl-2-Oleoyl-sn-Glycero-3-[Phospho-(1-glycerol)]; PHOSPHATIDYLGLYCEROL

Structure of a pore-blocking toxin in complex with a eukaryotic voltage-dependent K(+) channel.

Voltage-gated potassium channel subunit beta-2

Potassium voltage-gated channel subfamily A member 2, Potassium voltage-gated channel subfamily B member 1

RAK, RBK2, RCK5, Voltage-gated potassium channel subunit Kv1.2, Delayed rectifier potassium channel 1, DRK1, Voltage-gated potassium channel subunit Kv2.1

This is a chimeric channel between Kv1.2 and Kv2.1

PEG 400, POTASSIUM CHLORIDE, TRIS , pH 8.9, vapor diffusion, hanging drop, temperature 293K

Structure of the Kv1.2-2.1 paddle chimera channel with selenomethionine derivative of Charybdotoxin

ONE CHANNEL TETRAMER (GENERATED BY USING THE BIOMT TRANSFORMATIONS ON CHAINS G AND H AS NOTED ABOVE) BINDS TO ONE MOLECULE OF TOXIN (CHAIN Y). THE TOXIN CAN BIND IN FOUR DISTINCT ORIENTATIONS ALL OF WHICH ARE PARTIALLY OCCUPIED IN THE LATTICE. THE TOXIN WAS REFINED WITH 1/4 OCCUPANCY WITH ONE ORIENTATION OF THE TOXIN IN THE ASYMMETRIC UNIT. THE SYMMETRY OPERATIONS AROUND THE 4 FOLD SYMMETRY AXIS GENERATES THE OTHER POSSIBLE THREE ORIENTATIONS. PLEASE SEE PRIMARY CITATION FOR MORE DETAILS.
PROTEIN IN CHAINS B,H IS A CHIMERIC PROTEIN OF RAT KV1.2 AND RAT KV2.1. PLEASE REFER TO THE PRIMARY CITATION FOR MORE DETAILS.

THERE IS A TOXIN MOLECULE BOUND TO THE CHANNEL TETRAMER GENERATED BY FOUR COPIES OF A TOGETHER WITH FOUR COPIES OF B. HOWEVER IT WAS NOT BUILT BECAUSE IT WAS NOT SUFFICIENTLY WELL ORDERED.

RESIDUES 133-144 IN CHAIN B WAS BUILT AS A POLYGLYCINE CHAIN BECAUSE OF LACK OF ADEQUATE ELECTRON DENSITY FOR THE SIDE CHAINS.

Voltage-gated potassium channel subunit beta-2, Potassium voltage-gated channel subfamily A member 2, Potassium channel toxin alpha-KTx 1.1

potassium channel, pore blocking toxin, protein-protein complex, trans-enhanced dissociation effect, TRANSPORT PROTEIN-toxin complex