The crystal structure of the bacteriophage T4 MotA C-terminal domain in complex with dsDNA reveals a novel protein-DNA recognition motif

The phage T4 MotA transcription factor contains a novel DNA binding motif that specifically recognizes modified DNA.

non-native amino acids from expression vector= EGDIHM. N-TERM RESIDUES (93-96) = ELLK. LINKER (97-104) = KRATRKAR. HTTP://WWW.UNIPROT.ORG/UNIPROT/P22915
Middle transcription regulatory protein motA

DNA (5'-D(*GP*AP*AP*GP*CP*TP*TP*TP*GP*CP*TP*TP*AP*AP*TP*AP*AP*TP*CP*CP*AP*C)-3')

DNA (5'-D(*GP*TP*GP*GP*AP*TP*TP*AP*TP*TP*AP*AP*GP*CP*AP*AP*AP*GP*CP*TP*TP*C)-3')

23% PEG 8K, 0.1 M Na Acetate, 0.1 M NaCacodylate, pH 6.5, and 3% glycerol

The settings of PHENIX.REFINE were tuned to use reference model restraints (PDB: 1KAF), NCS and the twin law K,H,-L.

Middle transcription regulatory protein motA/DNA Complex

MotA, dsDNA, "Double wing", DNA binding motif, VIRAL PROTEIN-DNA complex