AGGLUTININ IN COMPLEX WITH T-DISACCHARIDE

Structure of benzyl T-antigen disaccharide bound to Amaranthus caudatus agglutinin.

Isolation and Characterization of Amaranthin, a Lectin Present in the Seeds of Amaranthus Caudatus, that Recognizes the T-(or Cryptic T)-Antigen

COORDINATES OF THE UNLIGANDED ACA STRUCTURE ARE IN PDB ENTRY 1JLY.

THE SEQUENCE WAS DEDUCED FROM THE KNOWN SEQUENCE OF AN APPARENT HOMOLOGUE FROM AMARANTHUS HYPOCHONDRIACUS (PIR 99583, 423770) REPORTED AS A "SEED SPECIFIC PROTEIN OF BALANCED NUTRITIONAL QUALITY" (RAINA,A. AND DATTA,A., PROC. NATL. ACAD. SCI. U.S.A. 89, 11774-11778 (1992)), PARTIAL SEQUENCES OF PEPTIDE FRAGMENTS DERIVED FROM THE AMARANTHUS CAUDATUS PROTEIN BY PROTEOLYSIS, AND THE EXPERIMENTAL ELECTRON DENSITY. IT IS ALSO PROBABLE THAT IN SOME (AS YET UNIDENTIFIED) POSITIONS, MORE THAN ONE RESIDUE TYPE CONTRIBUTES TO THE ELECTRON DENSITY. ONLY ONE RESIDUE TYPE PER POSITION HAS BEEN MODELLED.

THE N-TERMINAL ALA 1 APPEARS TO HAVE DENSITY CONSISTENT WITH A FORMYL GROUP ATTACHED TO THE N-TERMINUS.

COMPLEX (LECTIN-SACCHARIDE), T-DISACCHARIDE HOMODIMER, BIVALENT, LECTIN