Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Co ion

A conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligation.

PRP8 RNase H-like domain, UNP residues 1769-1990

220 kDa U5 snRNP-specific protein, PRP8 homolog, Splicing factor Prp8, p220

15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

metalloprotein, conformational change, RNA BINDING PROTEIN