A Structurally Dynamic Region of the HslU Intermediate Domain Controls Protein Degradation and ATP Hydrolysis.

Crystal structures of the HslVU peptidase-ATPase complex reveal an ATP-dependent proteolysis mechanism.

ATP-dependent protease ATPase subunit HslU

0.1 M Bis-Tris pH 5.5, 1.8 M Ammonium Sulfate, 7.5 mg/ml HslU(L199Q), 9.7 mg/ml HslV, 5 mM ATP, 0.5 mg/ml Arc-st11-ssrA (synthetic peptide substrate)

National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Current entry has a L199Q mutation in the HslU chains

ATP-dependent protease subunit HslV (E.C.3.4.25.2), ATP-dependent protease ATPase subunit HslU