The 2.2 A crystal structure of CYP154C5 from Nocardia farcinica in complex with androstenedione

Enzyme-substrate complex structures of CYP154C5 shed light on its mode of highly selective steroid hydroxylation.

0.2 M MgCHO2 in the reservoir, condition mixed 1:1 from protein stock and reservoir, cocrystallisation with steroid , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED

Cytochrom P450, steroid hydroxylating monooxygenase, steroid binding, oxidoreductase-substrate complex