Five of Five VHHs Neutralizing Poliovirus Bind the Receptor-Binding Site.

REFINEMENT PROTOCOL--flexible DETAILS--Using stereochemically and icosahedrally restrained maximum likelihood refinement in REFMAC5, a representative subset of the full atomic model with all neighbors present was built and refined to fit the corresponding subset of the experimental map. Portions of the model whose density resembled a structural homolog were identified and restrained to agree with the homolog. Detailed atomic models were constructed in areas of difference wherever the resolution of the map permitted. The Fourier-amplitude-weighted average cosine of the phase discrepancy was tracked.
ML agreement with Fourier amplitudes and phases

(Single particle details: The particles were processed using Frealign.) (Single particle--Applied symmetry: I)

60 nanobody VHH monomers bind to each poliovirion
Nanobody PVSS8A in complex with poliovirus P1/Mahoney

Gatan K2 operated in Super-resolution mode

Blotted for 4 seconds before plunging into liquid ethane (homemade plunger).

icosahedral asymmetric unit, std point frame

poliovirus, nanobodies, VHH, neutralizing antibodies, VIRUS-IMMUNE SYSTEM complex