Atomic model of the VipA/VipB, the type six secretion system contractile sheath of Vibrio cholerae from cryo-EM

Structure of the Type VI Secretion System Contractile Sheath.

Magnification was calibrated in the microscope using a gold lattice.
Iterative Real Space Helical Reconstruction

Vibrio cholerae VipA/VipB sheath in a contracted state

Operated in counting non-superresolution mode

holey carbon grids, Quantifoil 1.2/1.3, imaged over the holes

Plunged into liquid ethane (FEI VITROBOT MARK IV)

Vibrio cholerae O1 biovar El Tor str. N16961

VipA/B, contractile sheath of the type IV secretion system