Structure of T7 gatekeeper protein (gp11)

Structural characterization of the bacteriophage t7 tail machinery.

REFINEMENT PROTOCOL--rigid body DETAILS--One monomer was manually fitted and then the oligomer was generated using SITUS program

50 mM Tris-HCl, 10 mM MgCl2, 100 mM NaCl

T7 tail complex formed by proteins gp8, gp11 and gp12

R2/2 Quantifoil coated with a thin carbon layer

Samples were applied to grids for 1 minute, blotted and plunged into liquid ethane (LEICA EM CPC).
Samples were applied to grids for 1 minute, blotted and plunged into liquid ethane.

Bacteriophage, DNA ejection, tail complex, gatekeeper, VIRAL PROTEIN