Structure of a Plasmodium vivax apical membrane antigen 1-Fab F8.12.19 complex

Cross-Reactivity Studies of an Anti-Plasmodium Vivax Apical Membrane Antigen 1 Monoclonal Antibody: Binding and Structural Characterisation.

Crystal Structure of the Malaria Vaccine Candidate Apical Membrane Antigen 1

MONOCLONAL ANTIBODY ISOTYPE IS IGG1, KAPPA
FAB FRAGMENT OF MONOCLONAL ANTIBODY F8.12.19

PLASMODIUM VIVAX APICAL MEMBRANE ANTIGEN 1

ANTIGEN-BINDING FRAGMENT FAB, HEAVY CHAIN

ANTIGEN-BINDING FRAGMENT FAB, LIGHT CHAIN

PVAMA1 AND FAB-F8.12.19 WERE MIXED IN A 1:1 STOICHIOMETRIC RATIO AND LEFT TO INCUBATE FOR 4 HOURS AT ROOM TEMPERATURE TO FORM THE ANTIBODY-ANTIGEN COMPLEX BEFORE MIXING WITH CRYSTALLISATION SCREENING BUFFERS. CRYSTALS USED FOR DIFFRACTION MEASUREMENTS WERE OBTAINED AS FOLLOWS. THE CRYSTALLISATION BUFFER IN THE RESERVOIR COMPRISED 10% PEG 6000 AND 0.1 M SODIUM ACETATE BUFFERED TO PH 4.4. THE CRYSTALLISATION DROP WAS PREPARED BY ADDING 0.8 MICROLITRES OF THE CRYSTALLISATION BUFFER TO 0.8 MICROLITRES OF THE PVAMA1-FAB COMPLEX, GIVING A FINAL PROTEIN CONCENTRATION OF 3.9 MG/ML.

CRYSTAL STRUCTURE OF APICAL MEMBRANE ANTIGEN 1 FROM PLASMODIUM VIVAX

SHEET
THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN
ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW,
TWO SHEETS ARE DEFINED.

ENGINEERED RESIDUE IN CHAIN D, SER 136 TO ASN ENGINEERED RESIDUE IN CHAIN D, ASN 184 TO ASP ENGINEERED RESIDUE IN CHAIN D, ASN 399 TO GLU

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. FAB LIGHT AND HEAVY CHAINS ARE NUMBERED ACCORDING TO THE KABAT CONVENTION. ANTIGEN RESIDUES D43 TO D420 AND RESIDUES D455 TO D487 ARE DISSORDERED IN THE CRYSTAL STRUCTURE

APICAL MEMBRANE ANTIGEN 1, FAB FRAGMENT OF MONOCLONAL ANTIBODY F8.12.19

IMMUNOGLOBULIN DOMAIN, IMMUNOGLOBULIN C REGION, MEMBRANE, TRANSMEMBRANE, HYPOTHETICAL PROTEIN, ANTIBODY CROSS-REACTIVITY, SURFACE ACTIVE PROTEIN, MALARIA VACCINE CANDIDATE, APICAL MEMBRANE ANTIGEN 1, IMMUNE SYSTEM