HK97-like fold fitted into 3D reconstruction of bacteriophage CW02

Sequence and Structural Characterization of Great Salt Lake Bacteriophage CW02, a Member of the T7-Like Supergroup.

REFINEMENT PROTOCOL--Automatic rigid body DETAILS--C-alpha coordinates pertaining to the HK97-fold were separately fitted as rigid bodies into capsid hexamers or pentamers.

Particles were manually selected using X3D

1.35 M NaCl, 48 mM MgSO4-7H2O, 1 mM CaCl2, 2 mM Tris-Cl

Head and tail phage. Sample buffer solution contains 8% NaCl

200 mesh, holey-carbon-coated copper grid

Blotted for 4 seconds before plunging into liquid ethane (FEI Vitrobot MarK III)

MODELED SEQUENCE COMPRISES RESIDUES 182-380 OF MAJOR CAPSID PROTEIN FROM ENTEROBACTERIA PHAGE HK97 (UNP P49861).

icosahedral asymmetric unit, std point frame

halophage, bacteriophage HK97, bacteriophage T7, T7-like phage, turret, extremophile, Great Salt Lake, VIRUS