Crystal Structure Francisella tularensis histidine acid phosphatase complexed with phosphate

Crystal Structures of the histidine acid phosphatase from Francisella tularensis provide insight into substrate recognition.

Francisella tularensis subsp. holarctica

0.05 - 0.20 M Bis-Tris buffer pH 5.0 - 6.5, 17 -25 % PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K

D261A mutant protein complexed with substrate 3'-AMP

same protein complexed complexed with L(+)-tartrate

based on test set used for refinement of the tartrate complex of this enzyme

Histidine Acid Phosphatase, HAP, Hydrolase