Pseudomonas putida CBB5 NdmA with caffeine

Structural and Mechanistic Insights into Caffeine Degradation by the Bacterial N-Demethylase Complex.

0.2M ammonium citrate/citric acid pH 7.5, 15% w/v PEG 3350, 20% v/v 2-propanol

Methylxanthine N1-demethylase NdmA (E.C.1.14.13.178)

N-demethylase, Rieske oxygenase, non-heme iron center, caffeine degradation, METAL BINDING PROTEIN