Structure of head fiber and inner core protein gp22 of native bacteriophage P68

Structure and genome ejection mechanism ofStaphylococcus aureusphage P68.

The refinement was conducted on rigid body fitted major capsid proteins and Arstotzka proteins obtained from related structure of 6IAT; EMD-4438. The chains were fitted into hexon adjacent to portal of P68 capsid obtained from 5-fold symmetrized reconstruction. Subsequently, poly-alanine chains were manually built into densities corresponding to N-terminal part of head-fiber (chain IDs H,K,L) and C-terminal part of inner core protein (chain IDs X,Y,Z). The map was masked according to the related pdb in chimera, normalized and put into several rounds of a real space refinement in Phenix.

blot time 2s; blot force -2; 3.6 ul of sample

Poly alanine chain fitted to the density of head-fiber

Poly alanine chain fitted to the density of the inner core protein bound to hexon adjacent to portal

Major head protein, Arstotzka protein, Head fiber, inner core protein

structural protein, bacteriophage, head fiber, inner core protein