HMG-CoA reductase from pseudomonas mevalonii complexed with dithio-HMG-coa

(3S,5S,9R,21S)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9,21-tetrahydroxy-8,8,21-trimethyl-10,14-dioxo-19-thioxo-2,4,6-trioxa-18-thia-11,15-diaza-3,5-diphosphatricosan-23-oic acid 3,5-dioxide

A Novel Role for Coenzyme A during Hydride Transfer in 3-Hydroxy-3-methylglutaryl-coenzyme A Reductase.

3-hydroxy-3-methylglutaryl-coenzyme A reductase

1.2M ammonium sulfate, 75mM sodium citrate, 100 mM sodium ADA with microseeding, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

same protein and ligand reduced with NADH

same protein in non-productive complex with HMG-CoA and NAD.

same protein with NAD+ and mevalonate bound/

same protein with HMG-CoA bound but lysed during data collection

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

3-hydroxy-3-methylglutaryl-coenzyme A reductase (E.C.1.1.1.88)