Crystal structure of the catalytic domain of MMP-12 in complex with a selective sugar-conjugated triazole-linked carboxylate chelating water-soluble inhibitor (DC24).

Sugar-Based Arylsulfonamide Carboxylates as Selective and Water-Soluble Matrix Metalloproteinase-12 Inhibitors.

MME,Macrophage elastase,hME,Matrix metalloproteinase-12,MMP-12

Protein-ligand: hMMP12 F67D E219Q at 465 micro-M, 20 milli-M acetohydroxamic acid in 0.020M Tris-HCl, 3 milli-M CaCl2, 0.2M NaCl, pH 7.5, 0.2 milli-M inhibitor (DC24) 10% DMSO
Precipitant: 35% PEG4K, 0.2M imidazole piperidine, 15% dioxane, pH 8.5.
Cryoprotectant:
40% cryomix: (25 % diethylene glycol + 12.5 % glycerol + 25 % 1,2-propanediol + 12.5 % 1,4-dioxane) 25% PEG 6K, 100mM TRIS HCl, pH 8.0

Macrophage metalloelastase (E.C.3.4.24.65)

Inhibitor, complex, glycoconjugate, metalloprotease, hydrolase