Cocaine Esterase with mutation T172R, bound to DTT adduct

Structural analysis of thermostabilizing mutations of cocaine esterase.

10mM Tris pH 7.5, 25 mM NaCl, 1.6 M Ammonium Sulfate, 10mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Wild-type protein, in complex with transition state analog.

Wild-type protein, in complex with product.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS