Structure of the citryl-CoA lyase core module of Chlorobium limicola ATP citrate lyase (space group P21)

Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle.

Prior to crystallization, the N-terminal sequence MGSSHHHHHHSSGLVPR was removed by thrombin digestion.

22% PEG Smear Broad
0.1 M MES pH 6.5

Protein sample buffer
20 mM HEPES, pH 7.4, 150 mM NaCL supplemented with 50 mM citrate pH 7.4

ATP citrate lyase, central metabolism, acetyl-Coa, citrate shuttle, rTCA cycle, citryl-CoA, lipogenesis, LYASE