Non-covalent complex of and DAHP synthase and chorismate mutase from Corynebacterium glutamicum with bound transition state analog

8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC ACID

Inter-Enzyme Allosteric Regulation of Chorismate Mutase in Corynebacterium glutamicum: Structural Basis of Feedback Activation by Trp.

3-Deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase

100 mM imidazole/MES buffer, pH 6.5
30 mM each of ethylene glycol mix (di-ethylene glycol, tri-ethylene glycol, tetra-ethylene glycol, penta-ethylene glycol)
15% glycerol
15% PEG 4000

micro-seeded from badly diffracting crystals (approx. 8 AA resolution), in 100 mM Na-HEPES, pH 7.5, 200 mM LiSO4, 25% PEG 3350

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

3-Deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase (E.C.2.5.1.54), Chorismate mutase (E.C.5.4.99.5)

chorismate mutase, DAHP synthase, shikimate pathway, complex, Transferase/Isomerase, Transferase-Isomerase complex