Hepatitus C envelope glycoprotein E2 fragment 412-423 with humanized and affinity-matured antibody MRCT10.v362

Glycan shifting on hepatitis C virus (HCV) e2 glycoprotein is a mechanism for escape from broadly neutralizing antibodies.

COVALENT BOND LENGTH OF CB-SG ATOMS OF CYS A214 IS OUTSIDE OF NORMAL RANGE

LiCl, PEG6000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K

This sequence occurs naturally in hepatitus C virus

E2-PEPTIDE IS BIOTINYLATED, WITH BIOTIN (BTN) ATTACHED TO THE SIDE CHAIN OF THE C-TERMINAL LYS RESIDUE. HOWEVER, SOME RESIDUES AT THE C-TERMINUS, INCLUDING BTN, ARE MISSING DUE TO DISORDER.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

MRCT10.v362 Fab light chain, MRCT10.v362 Fab heavy chain, E2-peptide