Crystal structure of Cordyceps militaris IDCase in complex with uracil

Crystal structures of isoorotate decarboxylases reveal a novel catalytic mechanism of 5-carboxyl-uracil decarboxylation and shed light on the search for DNA decarboxylase.

25% PEG3350, 0.2 M magnesium chloride, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT U VALUES : REFINED INDIVIDUALLY

Uracil-5-carboxylate decarboxylase (E.C.4.1.1.66)

pyrimidine metabolism, DNA decarboxylation, IDCase, decarboxylase, uracil, LYASE